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This bret signal can be detected by using the deep blue coelenterazine (dbc) substrate for rluc. Here, we describe how recent improvements in detection devices and bret probes can be used to markedly improve the resolution of bret imaging, thus widening the. Bret 6 exhibited a bret efficiency of 83% with the shortest linker (fl 1), which decreased to 69% when the bret components were separated by the fl 9 linker (table s1).
These systems have revolutionized the way we.
Bret relies on forster resonance energy transfer from a. Previously described bret systems include bret 1 and bret 2, which use renilla luciferase (rluc) as bret donors [4].
